DETERMINATION, ISOLATION, AND IDENTIFICATION OF AUCUBIN AND VERBASCOSIDE IN THE LEAVES OF IRAQI PLANTAGO LANCOLETA L. USING DIFFERENT DETECTING METHOD S
By: Khalaf, Hasan A
.
Contributor(s): Ibrahim S. Abbas.
Publisher: M P Innovare Academic Sciences Pvt Ltd 2019Edition: Vol.11(2).Description: 74-80p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
International journal of pharmacy and pharmaceutical scienceSummary: Objective:
Plantago
lanceoleta
L. (ribwort plantain) is one of the important medicinal herbs which is widespread fortune available in Iraq, that have
a wide range of medicinal properties. The aim of this work
was
to determine, isolate and identify verbascoside and aucubin
in Iraqi
P
. lanceoleta
L.
by using d
ifferent chromatographic and spectrometric methods.
Method
s:
Verbascoside
and
aucubin
were isolated and quantified by preparative TLC,
and then
they were determined by the
high
-performance
thin
-layer chromatography
(HPTLC) fingerprinting. Aucubin and catalpol
in the plant extract were analyzed by
liquid
chromatography
-mass
spectrometry
(LC
-MS);
aucubin
and verbascoside that isolated from the plant sample were examined by f
ourier
-transform
infrared
spectroscopy
(FT
-IR) and LC
-MS, respectively.
Resul
ts:
The result showed that the Iraqi
P.
lanceoleta
L. contains 1.74 percent (verbascoside) and 0.24 percent (
aucubin
) of dry powdered leaves.
Each TLC
-isolated compound showed a single spot on the HPTLC plate, which
give
an
idea about the purity of the isolated compound. Aucubin (with
catalpol
) and verbascoside both are detected by LC
-MS in different ionization mode. Ma
ny functional groups were identified in the TLC
-isolated
aucubin
by FT
-IR.
Conclusio
n:
The Iraqi
P.
lanceoleta
L. showed a high content of verbasoside
, and it is a very rich source for this compound, which can be easily
isolated by TLC and subjected to many pharmacological studies. The extract of the young leaves of this plant gave a little am
ount of
aucubin
, and it is
easy to obtain a higher content fr
om the older leaves.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2020898 |
Objective:
Plantago
lanceoleta
L. (ribwort plantain) is one of the important medicinal herbs which is widespread fortune available in Iraq, that have
a wide range of medicinal properties. The aim of this work
was
to determine, isolate and identify verbascoside and aucubin
in Iraqi
P
. lanceoleta
L.
by using d
ifferent chromatographic and spectrometric methods.
Method
s:
Verbascoside
and
aucubin
were isolated and quantified by preparative TLC,
and then
they were determined by the
high
-performance
thin
-layer chromatography
(HPTLC) fingerprinting. Aucubin and catalpol
in the plant extract were analyzed by
liquid
chromatography
-mass
spectrometry
(LC
-MS);
aucubin
and verbascoside that isolated from the plant sample were examined by f
ourier
-transform
infrared
spectroscopy
(FT
-IR) and LC
-MS, respectively.
Resul
ts:
The result showed that the Iraqi
P.
lanceoleta
L. contains 1.74 percent (verbascoside) and 0.24 percent (
aucubin
) of dry powdered leaves.
Each TLC
-isolated compound showed a single spot on the HPTLC plate, which
give
an
idea about the purity of the isolated compound. Aucubin (with
catalpol
) and verbascoside both are detected by LC
-MS in different ionization mode. Ma
ny functional groups were identified in the TLC
-isolated
aucubin
by FT
-IR.
Conclusio
n:
The Iraqi
P.
lanceoleta
L. showed a high content of verbasoside
, and it is a very rich source for this compound, which can be easily
isolated by TLC and subjected to many pharmacological studies. The extract of the young leaves of this plant gave a little am
ount of
aucubin
, and it is
easy to obtain a higher content fr
om the older leaves.
Click on an image to view it in the image viewer
There are no comments for this item.